|
KMID : 0379520050210010031
|
|
한국독성학회지
2005 Volume.21 No. 1 p.31 ~ p.37
|
|
|
Protective Effect of the Stem Bark of Syringa velutina on Bisphenol-A in the Human Breast Cancer Cell Line and Immature Rat
|
|
Jo Eun-Hye
Yang Se-Ran
Cho Sung-Dae
Jung Ji-Won
Park Joon-Suk
Hwang Jae-Woong
Lee Sung-Hoon
Park Jung-Ran
Lee Yong-Soon
Kang Kyung-Sun
|
|
|
Abstract
|
|
|
|
The inhibitory activity against bisphenol-A (BPA), one of well-known endocrine disrupters was examined with the water extracts prepared from the Stem Bark of Syringa velutina (SBS). In this study, we have investigated the effect of SBS on the toxicity caused by BPA in human breast cancer cell line, MCF-7 cells and immature Sprague-Dawley rats. In the estrogen receptor-mediated proliferation assay using MCF-7 cells, BPA (16 ng/ml) induced the cell proliferation, but the water extract of SBS inhibited BPA-induced cell proliferation in a dose-dependent manner. These results are associated with PARP degradation and specific cleavage of anti-apoptotic protein Bcl-2 of apoptotic regulatory factors. Additionally, the BPA (400 mg/100 g) significantly induced the increase of the uterine and virginal weights, while SBS (50 mg/100 g) showed the inhibitory action against BPA, i.e. caused the increase of estrogen-related organ weights in immature rat uterotrophic assay. Taken together, the present data suggest that SBS may have anti-toxicity activities against BPA in vitro and in vivo systems. SBS may be capable of inhibiting adverse effects of BPA such as reproductive disorder.
|
|
|
KEYWORD
|
|
|
Bisphenol-A, MCF-7 cells, Syringa velutina, Uterotrophic assay, PARP, Bcl-2
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|
|